Reproductive Partners Fertility Blog » CGH http://www.reproductivepartners.com/blog Helping couples acheive the dream of being parents. Wed, 08 Sep 2010 22:30:51 +0000 http://wordpress.org/?v=2.9.2 en hourly 1 RPMG moves to CGH for PGS for chromosomes http://www.reproductivepartners.com/blog/383/rpmg-moves-to-cgh-for-pgs-for-chromosomes.html http://www.reproductivepartners.com/blog/383/rpmg-moves-to-cgh-for-pgs-for-chromosomes.html#comments Wed, 28 Apr 2010 00:20:13 +0000 Dr. Arthur Wisot FACOG http://www.reproductivepartners.com/blog/?p=383 RPMG will now be using comparative genomic hybridization (CGH) for preimplantation genetic screening (PGS) for chromosome screening of embryos (including for sex selection). CGH is a technique that involves amplifying the DNA and uses many thousands of probes to check all 23 chromosome pairs. As long as an intact nucleus is biopsied, it is virtually error-free, whereas with the…

]]> RPMG will now be using comparative genomic hybridization (CGH) for preimplantation genetic screening (PGS) for chromosome screening of embryos (including for sex selection). CGH is a technique that involves amplifying the DNA and uses many thousands of probes to check all 23 chromosome pairs. As long as an intact nucleus is biopsied, it is virtually error-free, whereas with the older technique, fluorescence in-situ hybridization (FISH), many errors may occur because FISH relies on binding of a single probe to each chromosome, and there are potential cell fixation artifacts, overlapping signals, and a subjective microscopic analysis is required. It also does not analyze all chromosomes. Unfortunately many normal embryos have been discarded with that technique due to these artifacts, and false negative results have also occurred where an embryo appeared normal but was actually abnormal.

For more information on PGS for chromosomes please check our our PGS for chromsomes page.

]]> http://www.reproductivepartners.com/blog/383/rpmg-moves-to-cgh-for-pgs-for-chromosomes.html/feed 0 Does preimplantation genetic screening (PGS) improve IVF success rates in women over 35? http://www.reproductivepartners.com/blog/338/does-preimplantation-genetic-screening-pgs-improve-ivf-success-rates-in-women-over-35.html http://www.reproductivepartners.com/blog/338/does-preimplantation-genetic-screening-pgs-improve-ivf-success-rates-in-women-over-35.html#comments Fri, 05 Mar 2010 18:56:46 +0000 Dr. Arthur Wisot FACOG http://www.reproductivepartners.com/blog/?p=338 Some fertility centers promote preimplantation genetic screening as a way of improving IVF success rates in women over 35 (defining advanced maternal age).

Now a study in the January 2010 edition of Fertility & Sterility shows that PGS does not significantly improve implantation, pregnancy or live birth rates. In this randomized control study from Belgium, the clinical implantation rate per…

]]> Some fertility centers promote preimplantation genetic screening as a way of improving IVF success rates in women over 35 (defining advanced maternal age).

Now a study in the January 2010 edition of Fertility & Sterility shows that PGS does not significantly improve implantation, pregnancy or live birth rates. In this randomized control study from Belgium, the clinical implantation rate per embryo transferred was compared between the PGS group (analysis of chromosomes 13, 16, 18, 21, 22, X, and Y by FISH) and the control group without PGS.

No differences were observed between the PGS group and the control group for the clinical implantation rate, the ongoing pregnancy rate at 12 weeks and the live born rate per embryo transferred.  A normal chromosome component was observed in only 30.3% of the embryos screened by PGS.

In this randomized controlled trial, the results did not confirm the hypothesis that PGS by FISH in 3-Day embryos results in improved reproductive outcome in patients with "advanced maternal age.".

In the future newer techniques of chromosome analysis such as comparative genomic hybridization (CGH) which can analyze all chromosome pairs and the ability to analyze blastocyst cells, freeze the embryos and transfer only chromosomally normal embryos may result in better outcomes and the need to transfer fewer embryos and reduce high-order multiple pregnancies as well.

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